Ph.D. Student
By Sarah Vos
photos by Mark Cornelison
On a recent Monday afternoon, Miranda Lange filled four small dishes, each containing a small rectangle of paper, with a buffer solution and set them on a rocking platform to gently wash an antibody away. The papers, really a membrane of mesh-like nitrocellulose, were covered with proteins extracted from the brains of transgenic mice, some of which have the mouse-equivalent of Alzheimer disease.
When the experiment is finished, Lange, using sophisticated proteomics methods, should be able to identify which of the brain proteins have suffered the most from oxidization. And in the case of the Alzheimer’s brain, oxidation is bad.
Lange, a fourth year graduate student at the University of Kentucky, has worked mainly on projects involving Alzheimer’s. In a previous project, she